A short duration pulse of polarized light is used to excite the sample. The decay of fluorescence is monitored first paralell (I||(t)), then perpendicular (I-(t)) to the excitation. From these measurements one can determine the decay of anisotropy r(t)
which procides information about the roatation of the fluorescing species.
LIGHT SOURCE
POLARIZER
DETECTOR
x
y
z
I
I
Anisotropy measurements allow one to determine how far a species rotates during its fluorescecne lifetime.
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